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Assessment of the biofilms produced by C. parapsilosis isolates. <t>Biofilm</t> optical density assay <t>on</t> <t>polystyrene</t> plates (A). Confocal scanning laser microscopy biofilm assay on silicone squares displaying top-views (upper panels) and side-views (lower panels) of mature biofilms (B); scale bars = 25 μm. Biofilm tolerance to 2.5% bleach (C) and 100 mM of hydrogen peroxide (D). Two-tailed T-tests were used to assess statistical significance and values ≤0.05, ≤ 0.01, and ≤0.001 are shown as *, **, and ****, respectively. S-Ege refers to susceptible isolates from EUH (1 T and 26 T), whereas Sx refers to susceptible isolate #314. G458S + R658G represents MDR0, Y132F + K143R + R658G features MDR1-4 and 6, Y132F + G307A + W1370R refers to MDR5 and 7, whereas ECR F652L represents the ECR isolates #315 and #316.
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Assessment of the biofilms produced by C. parapsilosis isolates. <t>Biofilm</t> optical density assay <t>on</t> <t>polystyrene</t> plates (A). Confocal scanning laser microscopy biofilm assay on silicone squares displaying top-views (upper panels) and side-views (lower panels) of mature biofilms (B); scale bars = 25 μm. Biofilm tolerance to 2.5% bleach (C) and 100 mM of hydrogen peroxide (D). Two-tailed T-tests were used to assess statistical significance and values ≤0.05, ≤ 0.01, and ≤0.001 are shown as *, **, and ****, respectively. S-Ege refers to susceptible isolates from EUH (1 T and 26 T), whereas Sx refers to susceptible isolate #314. G458S + R658G represents MDR0, Y132F + K143R + R658G features MDR1-4 and 6, Y132F + G307A + W1370R refers to MDR5 and 7, whereas ECR F652L represents the ECR isolates #315 and #316.
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Assessment of the biofilms produced by C. parapsilosis isolates. Biofilm optical density assay on polystyrene plates (A). Confocal scanning laser microscopy biofilm assay on silicone squares displaying top-views (upper panels) and side-views (lower panels) of mature biofilms (B); scale bars = 25 μm. Biofilm tolerance to 2.5% bleach (C) and 100 mM of hydrogen peroxide (D). Two-tailed T-tests were used to assess statistical significance and values ≤0.05, ≤ 0.01, and ≤0.001 are shown as *, **, and ****, respectively. S-Ege refers to susceptible isolates from EUH (1 T and 26 T), whereas Sx refers to susceptible isolate #314. G458S + R658G represents MDR0, Y132F + K143R + R658G features MDR1-4 and 6, Y132F + G307A + W1370R refers to MDR5 and 7, whereas ECR F652L represents the ECR isolates #315 and #316.

Journal: Emerging Microbes & Infections

Article Title: Evaluation of outbreak persistence caused by multidrug-resistant and echinocandin-resistant Candida parapsilosis using multidimensional experimental and epidemiological approaches

doi: 10.1080/22221751.2024.2322655

Figure Lengend Snippet: Assessment of the biofilms produced by C. parapsilosis isolates. Biofilm optical density assay on polystyrene plates (A). Confocal scanning laser microscopy biofilm assay on silicone squares displaying top-views (upper panels) and side-views (lower panels) of mature biofilms (B); scale bars = 25 μm. Biofilm tolerance to 2.5% bleach (C) and 100 mM of hydrogen peroxide (D). Two-tailed T-tests were used to assess statistical significance and values ≤0.05, ≤ 0.01, and ≤0.001 are shown as *, **, and ****, respectively. S-Ege refers to susceptible isolates from EUH (1 T and 26 T), whereas Sx refers to susceptible isolate #314. G458S + R658G represents MDR0, Y132F + K143R + R658G features MDR1-4 and 6, Y132F + G307A + W1370R refers to MDR5 and 7, whereas ECR F652L represents the ECR isolates #315 and #316.

Article Snippet: For the optical density (OD) biofilm assay, non-tissue culture treated sterile flat-bottomed 96-well polystyrene plates (Corning, Catalog #351143) were used to grow biofilms.

Techniques: Produced, Microscopy, Biofilm Production Assay, Two Tailed Test